Cathepsin D antibody (C-Term)

Catalog No. ABIN6254162

There is 1 publication for this product available. The Goat Polyclonal anti-Cathepsin D antibody is suitable to detect Cathepsin D in samples from Human, Mouse, Rat, Monkey, Hamster, Cow, Pig, Rabbit, Dog, Horse, Guinea Pig, Avian, Cat, Chicken, Donkey, Goat and Sheep. It has been validated for WB, IF, IHC (p), IHC (fro) and IEM.

Quick Overview for Cathepsin D antibody (C-Term) (ABIN6254162)

Target: Cathepsin D (CTSD)

Reactivity: Human, Mouse, Rat, Monkey, Hamster, Cow, Pig, Rabbit, Dog, Horse, Guinea Pig, Avian, Cat, Chicken, Donkey, Goat, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This Cathepsin D antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Product Details anti-Cathepsin D Antibody

Binding Specificity: C-Term

Purpose: Anti-Cathepsin D

Sequence: DQVEVASGLT LCKEGCEAIV DTGTSLMVGP VDEVRELQKA IGAVPLIQGE YMIPCEKVST LPAITLKLGG KGYKLSPEDY TLKVSQAGKT LCLSGFMGMD IPPPSGPLWI LGDVFIGRYY TVFDRDNNRV GFAEAARL

Specificity: This antibody gives a positive signal in the following human (Jurkat, HT1080, HUH, MDA-MB-231, ARPE19, SH-SY5Y), canine (MDCK) and monkey (COS-7) whole cell lysates.

Characteristics: Cathepsin D

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 275 aa to the C-terminus of human Cathepsin D produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:250-1:1,000, IF:1:50-1:200, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000, IEM:1:50-1:200

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-Cathepsin D antibody (ABIN6254162)

Pelkonen, Sato, Reinisalo, Kidron, Tachikawa, Watanabe, Uchida, Urtti, Terasaki: "LC-MS/MS Based Quantitation of ABC and SLC Transporter Proteins in Plasma Membranes of Cultured Primary Human Retinal Pigment Epithelium Cells and Immortalized ARPE19 Cell Line." in: Molecular pharmaceutics, Vol. 14, Issue 3, pp. 605-613, (2017) (PubMed).

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D

Background:

Goat polyclonal antibody to Cathepsin D. Cathepsin D is a lysosomal aspartic protease of the pepsin family. Acid protease active in intracellular protein breakdown. It is composed of a dimer of disulphide-linked heavy and light chains, both produced from a single protein precursor. It is involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

ceroid-lipofuscinosis, CLN10, CPSD, CTSD, lysosomal aspartyl protease, lysosomal aspartyl peptidase, neuronal 10 antibody.

Pathways: Peptide Hormone Metabolism