Keratin 5,6,18 antibody

Catalog No. ABIN6937074

The Mouse Monoclonal anti-Keratin 5,6,18 antibody is suitable to detect Keratin 5,6,18 in samples from Human, Rat and Cow. It has been validated for IHC.

Quick Overview for Keratin 5,6,18 antibody (ABIN6937074)

Target: Keratin 5,6,18

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Clone: LP34

Product Details

Purpose: Cytokeratin 5/6/18 Antibody

Specificity: It recognizes polypeptides of 58 kDa, 56 kDa, and 45 kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Detergent-insoluble fraction of psoriatic human epidermis

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HeLa, HepG2 or PC12 cells. Tonsil or Skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Keratin 5,6,18

Alternative Name: Cytokeratin 5/6/18

Background:

CK5, Keratin-5, KRT5, Cytoskeletal 6, KRT6, CK18, CYK18, K18, Keratin-18, KRT18

HeLa, HepG2 or PC12 cells. Tonsil or Skin.

Molecular Weight: 45.58kDa