DMC1 antibody

Catalog No. ABIN6937090

This anti-DMC1 antibody is a Mouse Monoclonal antibody detecting DMC1 in WB, IF and IHC (f). Suitable for Human.

Quick Overview for DMC1 antibody (ABIN6937090)

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DMC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 2H12-4

Product Details anti-DMC1 Antibody

Specificity: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the doublestrand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Nibrin and Dmc1. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients. Dmc1 is specifically involved in meiotic recombination. An alternative spliced form of Dmc1, designated Dmc1-D, is deleted for a region between the two motifs involved in nucleotide binding. The alternatively spliced Dmc1-D transcript is detected in both male and female germ cells, indicating that the encoded protein may have a role in mammalian genetic recombination in meiosis.

Cross-Reactivity (Details): Human. Mouse. Rat. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human DMC1 protein.

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Cervix or prostate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for DMC1

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Alternative Name: DMC1

Background: Disrupted meiotic cDNA 1 homolog, dJ199H16.1, DMC1 dosage suppressor of mck1 homolog, DMC1H, DNA meiotic recombinase 1, HsLim15, LIM15, Meiotic recombination protein DMC1/LIM15 homolog, MGC150472, MGC150473,DMC1
Cellular localisation: Nucleus. Chromosome.

Molecular Weight: 37kDa

Gene ID: 11144, 339396

UniProt: Q14565