Dystrophin antibody (AA 114-263)

Catalog No. ABIN6937091

This Mouse Monoclonal antibody specifically detects Dystrophin in IHC, ELISA, Coat and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Dystrophin antibody (AA 114-263) (ABIN6937091)

Target: Dystrophin (DMD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dystrophin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: DMD-3241

Product Details anti-Dystrophin Antibody

Binding Specificity: AA 114-263

Specificity: Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002 % of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa 114-263) of human DMD protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human skeletal muscle and heart muscle tissues (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Dystrophin

Target: Dystrophin (DMD)

Alternative Name: DMD

Background: BMD, CMD3B, Duchenne muscular dystrophy (DMD), Dystrophin, Muscular dystrophy Duchenne and Becker types,Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy)
Cellular localisation: Cell surface. Cytoplasm.

Molecular Weight: 427kDa

Gene ID: 1756, 495912

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development