ECM1 antibody

Catalog No. ABIN6937123

This anti-ECM1 antibody is a Mouse Monoclonal antibody detecting ECM1 in IF, FACS and IHC (f). Suitable for Human.

Quick Overview for ECM1 antibody (ABIN6937123)

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ECM1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SC05

Product Details anti-ECM1 Antibody

Specificity: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Cross-Reactivity (Details): Human and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full length native protein (purified) corresponding to Human IgA Secretory Component. Affinity-purified secretory component from human colostrum.

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Stomach, Lung, or Breast tumor.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ECM1

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Alternative Name: ECM1

Background: ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD,IgA Secretory Component / ECM1
Cellular localisation: Cell surface. Cytoplasm.

Molecular Weight: ~80kDa

Gene ID: 1893, 81071

UniProt: Q16610