EGFR antibody
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- Target See all EGFR Antibodies
- EGFR (Epidermal Growth Factor Receptor (EGFR))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EGFR antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin epiregulin.
- No Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Cross-Reactivity (Details)
- Human. Does not react with Mouse or Rat.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Purified EGFR from A431 cells.
- Clone
- H9B4
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product EGFR Primary Antibody
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1163-1191), (C-Term) antibodyEGFR Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal 688CT33-1-3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Mouse, Rat, Pig, Monkey, Cow, Chicken, Dog, Horse, Rabbit, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster, Sheep WB Host: Goat Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibodyEGFR Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Rat WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Known_Application: Immunoprecipitation (1-2 μg/500 μg protein lysate), Western Blot (1-2 μg/mL for 2 hours at RT), Optimal dilution for a specific application should be determined.
Positive_Control: A431 cells, Breast or Bladder cancer.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- EGFR (Epidermal Growth Factor Receptor (EGFR))
- Alternative Name
- EGFR (EGFR Products)
- Background
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Erbb1, ERBB1, Errp, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone, wa2, Wa5,EGFR (Epidermal Growth Factor Receptor)
Cellular localisation: Cell surface - Molecular Weight
- ~170kDa (wild type) and ~145kDa (vIII variant)
- Gene ID
- 1956, 488293
- UniProt
- P00533
- Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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