Ezrin antibody

Catalog No. ABIN6937202

This Mouse Monoclonal antibody specifically detects Ezrin in WB. It exhibits reactivity toward Human.

Quick Overview for Ezrin antibody (ABIN6937202)

Target: Ezrin (EZR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB)

Clone: CPTC-Ezrin-1

Product Details anti-Ezrin Antibody

Purpose: Ezrin / p81 Antibody

Specificity: Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70 % homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. Overexpression of Ezrin predicts the poor prognosis of gastric adenocarcinoma.

Cross-Reactivity (Details): Human. Others-not tested.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length Ezrin protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Western Blotting (1-2 μg/mL), ,Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SKBr3, HeLa, HEK293, MCF-7, Jurkat, A431, MCF-7. Cerebral cortex and hippocampus.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background:

Villin-2, CVIL, Epididymis secretory protein Li 105, EZR, p81, VIL2, Cytovillin, Cytovillin 2, HEL S 105, DKFZp762H157

A431, HEK293, HeLa, Jurkat, MCF-7, MCF-7. Cerebral cortex and hippocampus., SKBR3

Molecular Weight: 87kDa

Gene ID: 7430, 487027

UniProt: P15311

Pathways: Maintenance of Protein Location