Recombinant FTL antibody (AA 38-165)

Catalog No. ABIN6937272

The Rabbit Monoclonal anti-FTL antibody is suitable to detect FTL in samples from Human, Mouse, Rat, Rabbit, Guinea Pig and Hamster. It has been validated for WB and IHC.

Quick Overview for Recombinant FTL antibody (AA 38-165) (ABIN6937272)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FTL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: FTL-2338R

Product Details anti-FTL Antibody

Binding Specificity: AA 38-165

Purpose: Recombinant Ferritin, Light Chain (FTL) (Microglia Marker) Antibody

Specificity: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human FTL protein fragment (around aa 38-165) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Kidney, Cerebellum or Testis.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: FTL

Background:

Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3

Cerebellum or Testis., HeLa, HePG2, HL-60 or 293T cells. Pancreas, Kidney, Liver, Human Brain, Human Kidney, Human Liver, Human Lung, A431, Spleen

Molecular Weight: 19-25kDa

Gene ID: 2512, 433670

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis