CD15 antibody

Catalog No. ABIN6937284

The Rabbit Polyclonal anti-CD15 antibody is suitable to detect CD15 in samples from Human, Mouse and Rat. It has been validated for FACS, IHC and IF.

Quick Overview for CD15 antibody (ABIN6937284)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD15 Antibody

Purpose: CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody

Specificity: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 200ug/ml of Ab Purified by Protein A Column.

Immunogen: Purified human neutrophils

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml); Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: FUT4

Background:

3 Fucosyl N Acetyl Lactosamine; Alpha (1,3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1

Reed-Sternberg's cells in Hodgkin's lymphoma., U937 cells

Molecular Weight: 220kDa

Gene ID: 2526, 654379

UniProt: P22083