GFAP antibody (AA 101-200)

Catalog No. ABIN6937311

The Mouse Monoclonal anti-GFAP antibody is suitable to detect GFAP in samples from Human. It has been validated for WB and IHC.

Quick Overview for GFAP antibody (AA 101-200) (ABIN6937311)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: GFAP-2076

Product Details anti-GFAP Antibody

Binding Specificity: AA 101-200

Purpose: GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

Specificity: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human GFAP protein fragment (around aa 101-200) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

T98G cells (FACS). Human brain lysate (WB). Brain or Astrocytoma (IHC).

Molecular Weight: ~50kDa

Gene ID: 2670, 514227

UniProt: P14136