HLA-DPB1 antibody

Catalog No. ABIN6937401

The Mouse Monoclonal anti-HLA-DPB1 antibody is suitable to detect HLA-DPB1 in samples from Human. It has been validated for ELISA, FACS and IF.

Quick Overview for HLA-DPB1 antibody (ABIN6937401)

Target: HLA-DPB1 (Major Histocompatibility Complex, Class II, DP beta 1 (HLA-DPB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DPB1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BRA-FB6

Product Details anti-HLA-DPB1 Antibody

Purpose: HLA-DP (MHC II) Antibody

Specificity: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating use Ab at 2-4 μg/mL, order Ab without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA-DPB1

Target: HLA-DPB1 (Major Histocompatibility Complex, Class II, DP beta 1 (HLA-DPB1))

Alternative Name: HLA-DPB1

Background:

HLA class II histocompatibility antigen DP alpha 1 chain, HLA DP1A, HLA SB alpha chain, HLADP, HLADPA1, HLASB, HLASB histocompatibility type, Human MHC class II HLA SB alpha, Major histocompatibility complex class II DP alpha 1, MHC class II DP3 alpha, MHC class II DPA1, PLT1, Primed lymphocyte test 1

Raji cells. Tonsil or lymph node.

Molecular Weight: 33-35kDa

Gene ID: 3115, 347270

UniProt: P04440

Pathways: TCR Signaling, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response