ICAM1 antibody

Catalog No. ABIN6937462

This anti-ICAM1 antibody is a Mouse Monoclonal antibody detecting ICAM1 in FACS, IF, Func and IHC (f). Suitable for Human.

Quick Overview for ICAM1 antibody (ABIN6937462)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: W-CAM-1-same as Wehi-CAM-1 or 1H4

Product Details anti-ICAM1 Antibody

Specificity: Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of Purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Raji Burkitt lymphoma cells

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM1

Background: CD54, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM-1, Intercellular adhesion molecule 1, Ly 47, Major group rhinovirus receptor, MALA2, MyD10, Surface antigen of activated B cells,CD54 / ICAM-1
Cellular localisation: Cell Surface

Molecular Weight: 85-115kDa

Gene ID: 3383, 643447

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling