IgD antibody

Catalog No. ABIN6937493

This Mouse Monoclonal antibody specifically detects IgD in FACS, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for IgD antibody (ABIN6937493)

Target: IgD

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgD antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: IgD26

Product Details anti-IgD Antibody

Purpose: IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker) Antibody

Specificity: Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin D (IgD) exists as a monomer with light chains. It plays a biological role as a transmembrane receptor molecule, co-expressed with IgM on the surface of mature/naive B cells. In particular, it is found on spleen B cell surfaces. Compared to IgM, IgD exists in much lower numbers and is not expressed on immature B cells. IgD surface expression on B cells is regulated in part by IL-27. In mice, the inhibition of this immunoglobulin isotype does not cause a significant change to the immune system.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Full-length human IGHD protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil lysate, NAMALWA cell lysate, Human tonsil and lymphoid tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IgD

Target: IgD

Alternative Name: IGHD

Target Type: Antibody

Background:

Ig delta chain C region, IGHD, Immunoglobulin heavy constant delta

Human tonsil lysate, NAMALWA cell lysate, Human tonsil and lymphoid tissues.

Molecular Weight: 44-80kDa (heavy chain), 21-25kDa (kappa light chain)

Gene ID: 3495, 510635

UniProt: P01880