IGHG antibody

Catalog No. ABIN6937494

The Mouse Monoclonal anti-IGHG antibody is suitable to detect IGHG in samples from Human. It has been validated for FACS, IF and IHC (f).

Quick Overview for IGHG antibody (ABIN6937494)

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHG antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: IG217-IG266

Product Details anti-IGHG Antibody

Specificity: This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Gamma heavy chain(IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified human IgG

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IGHG

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Alternative Name: IGHG

Background: G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4,Immunoglobulin Gamma (IgG) Heavy Chain
Cellular localisation: Cytoplasm, Cell Surface and Secreted

Molecular Weight: ~75kDa

Gene ID: 3500, 3501, 3502

UniProt: P01857, P01859, P01860, P01861