IGHG antibody

Catalog No. ABIN6939711

Product Details anti-IGHG Antibody

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHG antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM), Immunofluorescence (IF)

Specificity: Recognizes a protein of 75 kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: Purified by Protein A/G

Immunogen: Purified human Ig Gamma Chain

Clone: SPM556

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHG

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Alternative Name: IGHG (IGHG Products)

Synonyms: AU044919 antibody, IGHG2A antibody, Immunoglobulin heavy chain (gamma polypeptide) antibody, Ighg antibody

Molecular Weight: 75kDa

Gene ID: 3500, 3501, 3502

UniProt: P01857, P01859, P01860, P01861