IGHG4 antibody

Catalog No. ABIN6937504

The Mouse Monoclonal anti-IGHG4 antibody (Clone IGHG4-1345) (ABIN6937504) specifically detects IGHG4 in IHC. The antibody is reactive with Human samples.

Quick Overview for IGHG4 antibody (ABIN6937504)

Target: IGHG4 (Immunoglobulin Heavy Constant gamma 4 (G4m Marker) (IGHG4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHG4 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: IGHG4-1345

Product Details anti-IGHG4 Antibody

Purpose: IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker) Antibody

Specificity: The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells.IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human IGHG4 fragment

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHG4

Target: IGHG4 (Immunoglobulin Heavy Constant gamma 4 (G4m Marker) (IGHG4))

Alternative Name: IGHG4

Background:

Ig gamma 4 chain C region, IGHG4, Immunoglobulin heavy constant gamma 4 (G4m marker)

Tonsil.

Molecular Weight: 150kDa

Gene ID: 3503, 510635

UniProt: P01861