IgL antibody

Catalog No. ABIN6937544

This Mouse Monoclonal antibody specifically detects IgL in FACS, IF, WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for IgL antibody (ABIN6937544)

Target: IgL

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HP6054

Product Details anti-IgL Antibody

Specificity: This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified human IgG myeloma proteins coupled to polyaminostyren microbeads

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IgL

Target: IgL

Background: Bence Jones Protein, BJP, IGLC 1/2/3, Mcg Marker, Paraprotein,Lambda Light Chain (B-Cell Marker)
Cellular localisation: Cell Surface, cytoplasmic and secreted

Molecular Weight: ~22.5kDa

Gene ID: 3535, 3546, 449585

UniProt: P01701, P01842