Recombinant IgL antibody

Catalog No. ABIN6939756

This Mouse Monoclonal antibody specifically detects IgL in IHC and WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant IgL antibody (ABIN6939756)

Target: IgL

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgL antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RLLC-3777

Product Details anti-IgL Antibody

Purpose: Recombinant Lambda Light Chain (B-Cell Marker) Antibody

Specificity: This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human IGL protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IgL

Target: IgL

Background:

Bence Jones Protein, BJP, IGLC 1/2/3, Mcg Marker, Paraprotein

293T, Raji or hPBL cells. Tonsil or Spleen. Human Serum.

Molecular Weight: ~22.5kDa

Gene ID: 3535, 3546, 449585

UniProt: P01701