Recombinant Integrin beta 3 antibody (AA 385-490)

Catalog No. ABIN6937603

This anti-Integrin beta 3 antibody is a Rabbit Monoclonal antibody detecting Integrin beta 3 in IHC (f). Suitable for Human.

Quick Overview for Recombinant Integrin beta 3 antibody (AA 385-490) (ABIN6937603)

Target: Integrin beta 3 (ITGB3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ITGB3-2166R

Product Details anti-Integrin beta 3 Antibody

Binding Specificity: AA 385-490

Specificity: Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61).The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human ITGB3 protein fragment (aa 385-490) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U937, KG1a, HEL cells. Spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: ITGB3

Background: BDPLT2, GP3A, GPIIIa, GT, HPA 1, HPA 4, Integrin beta-3, ITGB3, NAIT, Platelet fibrinogen receptor beta subunit, Platelet glycoprotein IIIa, PTP,CD61 / Integrin 3 / Platelet Glycoprotein IIIa (Platelet Marker)
Cellular localisation: Cell Surface

Molecular Weight: 105kDa & 90kDa

Gene ID: 3690, 218040

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex