Recombinant KRT20 antibody (AA 196-323)

Catalog No. ABIN6937708

The Rabbit Monoclonal anti-KRT20 antibody (Clone KRT20-3129R) (ABIN6937708) specifically detects KRT20 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant KRT20 antibody (AA 196-323) (ABIN6937708)

Target: KRT20 (Keratin 20 (KRT20))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: KRT20-3129R

Product Details anti-KRT20 Antibody

Binding Specificity: AA 196-323

Purpose: Recombinant Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Antibody

Specificity: This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HT29 cell lysate (WB). Human colon carcinoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: KRT20

Background:

CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20

HT29 cell lysate (WB). Human colon carcinoma (IHC).

Molecular Weight: 46kDa

Gene ID: 54474, 84905

UniProt: P35900