Keratin 3 antibody

Catalog No. ABIN6937709

This Mouse Monoclonal antibody specifically detects Keratin 3 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Keratin 3 antibody (ABIN6937709)

Target: Keratin 3 (KRT3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 3 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT3-2130

Product Details anti-Keratin 3 Antibody

Specificity: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins, which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human KRT3 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Cornea or Skin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Keratin 3

Target: Keratin 3 (KRT3)

Alternative Name: KRT3

Background: 65 kDa cytokeratin, CK3, Cytokeratin-3, K3, Keratin type II Cytoskeletal-3, Keratin-3, KRT3,Cytokeratin 3 (KRT3) (Corneal Epithelial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 65kDa

Gene ID: 38550, 680652

UniProt: P12035