Cytokeratin 2 antibody

Catalog No. ABIN6937731

The Mouse Monoclonal anti-Cytokeratin 2 antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect Cytokeratin 2 in samples from Human.

Quick Overview for Cytokeratin 2 antibody (ABIN6937731)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: DE-SQ

Product Details anti-Cytokeratin 2 Antibody

Specificity: This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC12 cells. Tonsil, Skin, Prostate, Esophagus, Ectocervix or Squamous carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Alternative Name: KRT76

Background: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
Cellular localisation: Cytoplasmic

Molecular Weight: 50-67kDa

Gene ID: 51350, 654392

UniProt: Q01546