Blood Group Lewis Y antibody

Catalog No. ABIN6937772

This Mouse Monoclonal antibody specifically detects Blood Group Lewis Y in FACS, IF, IHC and StM. It exhibits reactivity toward Human.

Quick Overview for Blood Group Lewis Y antibody (ABIN6937772)

Target: Blood Group Lewis Y

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: A70-A-A9

Product Details

Specificity: This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

Purification: Purified by Protein A/G

Immunogen: Live Ls174T cells (human colon carcinoma cell line)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Lung, Colon, Bladder or Hepatocellular Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Blood Group Lewis Y

Alternative Name: Lewis Y (Tumor Marker)