MBD1 antibody

Catalog No. ABIN6937807

The Mouse Monoclonal anti-MBD1 antibody (Clone CPTC-MBD1-1) (ABIN6937807) specifically detects MBD1 in IHC. The antibody is reactive with Human samples.

Quick Overview for MBD1 antibody (ABIN6937807)

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBD1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CPTC-MBD1-1

Product Details anti-MBD1 Antibody

Purpose: MBD1 (Methyl-CpG Binding Domain Protein 1) Antibody

Specificity: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has also been predicted to be involved in the development and progression of tumors, and it has been shown that MBD1 is significantly upregulated in pancreatic cancer tissues when compared with surrounding normal tissues. Furthermore, MBD1 may serve as a potential therapeutic target for pancreatic cancer.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human MBD1 protein

Isotype: IgG2, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil or colon (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: MBD1

Background:

CXXC-type zinc finger protein 3, CXXC3, MBD1, MECP1 COMPLEX, Methyl-CpG-binding domain protein 1 (MBD1), Protein containing methyl-CpG-binding domain 1 (PCM1), Regulator of fibroblast growth factor 2 (FGF 2) transcription (RFT)

Human tonsil or colon (IHC).

Molecular Weight: ~80kDa

Gene ID: 4152, 405610

UniProt: Q9UIS9