Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody

Catalog No. ABIN6937832

This Mouse Monoclonal antibody specifically detects Melanoma Marker (MART-1 + Tyrosinase + Gp100) in IHC. It exhibits reactivity toward Human.

Quick Overview for Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody (ABIN6937832)

Target: Melanoma Marker (MART-1 + Tyrosinase + Gp100)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Clone: DT101-BC199-T311-HMB45

Product Details

Purpose: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody

Specificity: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant hMART-1 protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45); Recombinant tyrosinase protein (T311)

Isotype: IgG1, IgG2a, IgG2b, kappa, lambda

Application Details

Application Notes:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Melanoma Marker (MART-1 + Tyrosinase + Gp100)

Background:

Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, PMEL17

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Molecular Weight: 20-22kDa (doublet) (MART-1): 90-100kDa (gp100), 70-80kDa (Tyrosinase)

Gene ID: 2315, 6490