Moesin antibody

Catalog No. ABIN6937914

The Mouse Monoclonal anti-Moesin antibody is suitable to detect Moesin in samples from Human. It has been validated for WB, IHC, IF and FACS.

Quick Overview for Moesin antibody (ABIN6937914)

Target: Moesin (MSN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MSN-493

Product Details anti-Moesin Antibody

Purpose: Moesin Antibody

Specificity: Recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Human. Does not react with Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Moesin protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562, HT-29, Jurkat, CH3LC or HUVEC cells. Uterus, placenta, tonsil (both B and T lymphocytes), skeletal muscle, thyroid or kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: MSN

Background:

Membrane-organizing extension spike protein, Moesin/anaplastic lymphoma kinase fusion protein, MSN/ALK fusion

CH3LC or HUVEC cells. Uterus, HT-29, Jurkat, K562, Placenta, Skeletal muscle, thyroid or kidney., tonsil (both B and T lymphocytes)

Molecular Weight: 78kDa

Gene ID: 4478, 87752

UniProt: P26038

Pathways: Asymmetric Protein Localization