MTF1 antibody

Catalog No. ABIN6937919

Product Details anti-MTF1 Antibody

Target: MTF1 (Metal-Regulatory Transcription Factor 1 (MTF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MTF1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The metal-responsive element (MRE)-binding transcription factor (MTF-1) stimulates the expression of metallothioneins in response to the exposure of cells to heavy metals. MTF-1 contains six zinc fingers in the DNA binding domain. The phosphorylation of MTF-1 in response to metal exposure appears to play a significant role in the ability of MTF-1 to activate metallothionein transcription. In addition to its role in metallothionein activation, MTF-1 is involved in a post-transcription regulatory complex for ribosomal protein S25. MTF-1, La and p53 inhibit the nuclear export of S25 mRNA in response to nutrient depravation. Furthermore, MTF-1 acts as a chromatin insulator on integrated transgenes in cultured cells to insulate active loci against chromatin silencing.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MTF1 protein

Clone: MTF1-2649

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MTF1

Target: MTF1 (Metal-Regulatory Transcription Factor 1 (MTF1))

Alternative Name: MTF1 (MTF1 Products)

Synonyms: MTF-1 antibody, MTF1 antibody, mtf1 antibody, MGC86485 antibody, Thyls antibody, ZRF antibody, metal regulatory transcription factor 1 antibody, metal-regulatory transcription factor 1 antibody, metal-regulatory transcription factor 1 L homeolog antibody, metal response element binding transcription factor 1 antibody, MTF1 antibody, mtf1 antibody, mtf1.L antibody, Mtf1 antibody

Background: Metal regulatory transcription factor 1, Metal response element binding transcription factor 1, Metal responsive transcription factor 1, MRE-binding transcription factor 1, MTF1, Transcription factor MTF1, Zinc regulatory factor (ZRF),MTF1
Cellular localisation: Nucleus.

Molecular Weight: 70kDa

Gene ID: 4520, 471991

UniProt: Q14872