MUC2 antibody

Catalog No. ABIN6937947

The Mouse Monoclonal anti-MUC2 antibody has been validated for IHC, IF, FACS and IHC (f). It is suitable to detect MUC2 in samples from Human.

Quick Overview for MUC2 antibody (ABIN6937947)

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MLP-842

Product Details anti-MUC2 Antibody

Specificity: Recognizes a single glycoprotein of 520 kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon, in about 65 % of colonic carcinomas and about 40 % of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Human. Does not react with rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment covering tandem repeats of human MUC2 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: LS174T cells. Human small intestine (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MUC2

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Alternative Name: MUC2

Background: Mucin-2, Intestinal mucin-2, Mucin like protein, (MLP), SMUC,MUC2 (Mucin 2)
Cellular localisation: Cytoplasmic and cell surface

Molecular Weight: 520kDa

Gene ID: 4583, 315

UniProt: Q02817