MVP antibody

Catalog No. ABIN6937973

This Mouse Monoclonal antibody specifically detects MVP in IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for MVP antibody (ABIN6937973)

Target: MVP (Major Vault Protein (MVP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 1032

Product Details anti-MVP Antibody

Purpose: Major Vault Protein (MVP) Antibody

Specificity: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: proteins precipitated from human breast cancer MCF-7 cells

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HeLa cells. Breast tumors.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: MVP

Background:

Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

MCF-7 or HeLa cells. Breast tumors.

Molecular Weight: 104-110kDa

Gene ID: 9961, 632177

UniProt: Q14764