Nestin antibody (AA 698-844)

Catalog No. ABIN6938043

The Mouse Monoclonal anti-Nestin antibody has been validated for IHC (f). It is suitable to detect Nestin in samples from Human.

Quick Overview for Nestin antibody (AA 698-844) (ABIN6938043)

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NES-2911

Product Details anti-Nestin Antibody

Binding Specificity: AA 698-844

Specificity: Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human NES protein fragment around aa 698-844 (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human kidney, ovary, cervix, breast, colon, endometrium, cerebellum, lung, prostate, skin and human melanoma tissue. HeLa cell lysate. SH-SY5Y cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: NES

Background: ESTM 46, FLJ21841, Intermediate filament protein, Nbla00170, nes,Nestin (Cancer Stem Cell Marker)
Cellular localisation: Cytoplasm and membrane.

Molecular Weight: 190-200kDa

Gene ID: 10763, 527971

UniProt: P48681