NFE2L1 antibody

Catalog No. ABIN6938044

This anti-NFE2L1 antibody is a Mouse Monoclonal antibody detecting NFE2L1 in IHC (f). Suitable for Human.

Quick Overview for NFE2L1 antibody (ABIN6938044)

Target: NFE2L1 (Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFE2L1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NRF1-2608

Product Details anti-NFE2L1 Antibody

Specificity: The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human NRF1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Ubiquitous expression, strongest in skeletal muscle.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NFE2L1

Target: NFE2L1 (Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1))

Alternative Name: NFE2L1

Background: Alpha pal, Alpha palindromic-binding protein, Alpha-pal, locus control region factor 1, NFE2 related factor 1, Nuclear respiratory factor 1 (NFR-1),NRF1
Cellular localisation: Nucleus.

Molecular Weight: 30kDa (bZIP region), 65-120kDa (glycosylated)

Gene ID: 4899, 654363

UniProt: Q16656