Recombinant NGFR antibody

Catalog No. ABIN6938052

The Rabbit Monoclonal anti-NGFR antibody has been validated for IHC (f) and Diff. It is suitable to detect NGFR in samples from Human.

Quick Overview for Recombinant NGFR antibody (ABIN6938052)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Differentiation (Diff)

Clone: NGFR-1997R

Product Details anti-NGFR Antibody

Specificity: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Human and Non-Human Primates. Does not react with mouse and rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length p75 NGFR protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Neuronal axons, Schwann cells, and perineural cells of peripheral nerves, or tumors of nerve sheath differentiation e.g. Schwannoma, Neurofibroma. Soma and axons of sensory neurons, and ganglionic satellite cells. Melanomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background: CD271, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR,), p75 ICD, p75 Neurotrophin receptor, Tumor necrosis factor receptor superfamily member 16 (TNFRSF16),NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker)
Cellular localisation: Cell surface. Cytoplasm.

Molecular Weight: 75kDa

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding