CD31 antibody

Catalog No. ABIN6938149

The Mouse Monoclonal anti-CD31 antibody (Clone SPM122) (ABIN6938149) specifically detects CD31 in FACS, WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for CD31 antibody (ABIN6938149)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: SPM122

Product Details anti-CD31 Antibody

Purpose: CD31 / PECAM-1 (Endothelial Cell Marker) Antibody

Specificity: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

No Cross-Reactivity: Pig (Porcine), Rat (Rattus)

Cross-Reactivity (Details): Human. Cynomolgus Monkey. Rabbit. Does not react with Rat or Pig.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Membrane preparation of a spleen from a patient with hairy cell leukemia

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil, Angiosarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM1

Background:

EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA'

Jurkat cells. Human tonsil.

Molecular Weight: ~100kDa (endothelium) and ~130kDa (platelets)'

Gene ID: 5175, 376675, 514412

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization