POLR2A/RPB1 antibody (pSer5)

Catalog No. ABIN6938207

This Mouse Monoclonal antibody specifically detects POLR2A/RPB1 in WB, IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for POLR2A/RPB1 antibody (pSer5) (ABIN6938207)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CTD4H8

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: pSer5

Specificity: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A

Background: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit, DNA-directed RNA polymerase II subunit RPB1, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa, RNA polymerase II subunit B1, RPBh1, RpIILS, RPO2, RPOL2,RNA Polymerase II
Cellular localisation: Nuclear

Molecular Weight: 192-253kDa

Gene ID: 5430, 270017

UniProt: P24928

Pathways: Regulatory RNA Pathways