POU2AF1 antibody (AA 148-255)

Catalog No. ABIN6938221

The Mouse Monoclonal anti-POU2AF1 antibody (Clone BOB1-2421) (ABIN6938221) specifically detects POU2AF1 in WB, IHC and FACS. The antibody is reactive with Human samples.

Quick Overview for POU2AF1 antibody (AA 148-255) (ABIN6938221)

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POU2AF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: BOB1-2421

Product Details anti-POU2AF1 Antibody

Binding Specificity: AA 148-255

Purpose: BOB.1 (B-Cell Marker) Antibody

Specificity: BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Raji, Ramos cells. Spleen or Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Alternative Name: POU2AF1

Background:

B cell Oct binding protein 1, B-cell-specific coactivator OBF-1, BOB-1, OBF1, OCA-B, OCAB, OCT-binding factor 1, POU domain class 2-associating factor 1, POU2AF1

Raji, Ramos cells. Spleen or Tonsil.

Molecular Weight: 35kDa

Gene ID: 5450, 654525

UniProt: Q16633