Proliferation Marker antibody

Catalog No. ABIN6938249

Product Details

Target: Proliferation Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Pokeweed-stimulated lymphocytes

Clone: JC1

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa cells (IF). Human lymph node (IHC).

Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Proliferation Marker

Background: JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

Molecular Weight: 212kDa and 123kDa