Proliferation Marker antibody

Catalog No. ABIN6938249

This Mouse Monoclonal antibody specifically detects in IHC, WB, IF and StM. It exhibits reactivity toward Human.

Quick Overview for Proliferation Marker antibody (ABIN6938249)

Target: Proliferation Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Staining Methods (StM)

Clone: JC1

Product Details

Purification: Purified by Protein A/G

Immunogen: Pokeweed-stimulated lymphocytes

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa cells (IF). Human lymph node (IHC).

Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Proliferation Marker

Background: JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

Molecular Weight: 212kDa and 123kDa