S100A8 antibody

Catalog No. ABIN6938338

This anti-S100A8 antibody is a Mouse Monoclonal antibody detecting S100A8 in IF, FACS and IHC (f). Suitable for Human.

Quick Overview for S100A8 antibody (ABIN6938338)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MRP8 7C12-4

Product Details anti-S100A8 Antibody

Specificity: Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Cross-Reactivity (Details): Human. Baboon. Monkey. Cow. Pig. Goat. Horse. Cat. Dog. Rabbit. Guinea Pig. Rat. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Affinity Purified monocyte membrane preparation

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil, lymph node or spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8

Background: 60B8AG, Calgranulin A (CAGA), Calgranulin B (CAGB), Calprotectin L1L subunit, Chemotactic cytokine CP-10, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, L1Ag, Macrophage L1 protein Calprotectin, Migration inhibitory factor related protein 8, Myeloid-related protein 8 (MRP-8 or P8), Myeloid-related protein 14 (MRP-14 or P14), Neutrophil cytosolic 7 kDa protein, NIF, S100A8/S100A9 complex, MA387, Pro-inflammatory S100 cytokine, Urinary stone protein band A,Calprotectin / MRP14 / S100A9 / Calgranulin B
Cellular localisation: Cytoplasmic

Molecular Weight: MAC387

Gene ID: 6279, 6280, 416073

UniProt: P05109, P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins