Recombinant SOX10 antibody (AA 115-269)

Catalog No. ABIN6938409

The Rabbit Monoclonal anti-SOX10 antibody has been validated for WB and IHC (f). It is suitable to detect SOX10 in samples from Human.

Quick Overview for Recombinant SOX10 antibody (AA 115-269) (ABIN6938409)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SOX10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SOX10-2311R

Product Details anti-SOX10 Antibody

Binding Specificity: AA 115-269

Specificity: SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Cross-Reactivity (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa115-269) of human SOX10 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Western Blot (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: COLO-38 or HepG2 cells. Melanomas, breast carcinomas, gliomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10

Background: DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E(WS2E), WS4, Waardenburg syndrome type 4C (WS4C),SOX10 (Melanoma Marker)
Cellular localisation: Nuclear

Molecular Weight: 49-58kDa

Gene ID: 6663, 376984

UniProt: P56693

Pathways: Chromatin Binding