Recombinant SOX9 antibody

Catalog No. ABIN6938417

This Mouse Monoclonal antibody specifically detects SOX9 in WB, IP and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant SOX9 antibody (ABIN6938417)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RSOX9-2288

Product Details anti-SOX9 Antibody

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTMArray, containing more than 19,000, full-length human proteins. Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Nucleus (Potential). Campomelic dysplasia (CMD1) [MIM:114290]: Rare, often lethal, dominantly inherited, congenital osteo-chondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognathia, flat face and hypertelorism are common.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length SOX9 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunoprecipitation (2-4 μg/mg protein),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human skin hair follicles.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background: CMD 1, campomelic dysplasia autosomal sex reversal, SRA1, SRXX2, SRXY10, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX 9,SOX9 / SRY-box 9
Cellular localisation: Nucleus.

Molecular Weight: 56kDa

Gene ID: 6662, 647409

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development