Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody
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- Target See all Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) Antibodies
- Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
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Binding Specificity
- AA 356-475
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)
- Clone
- SPTBN2-1582
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product SPTBN2 Primary Antibody
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- Application Notes
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells), (Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Pancreas or Liver.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
- Alternative Name
- SPTBN2 (SPTBN2 Products)
- Synonyms
- Spnb3 antibody, mKIAA0302 antibody, GTRAP41 antibody, SCA5 antibody, spectrin beta, non-erythrocytic 2 antibody, spectrin, beta, non-erythrocytic 2 antibody, Sptbn2 antibody, SPTBN2 antibody
- Background
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Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2,Spectrin beta III (SPTBN2)
Cellular localisation: Cell Surface - Molecular Weight
- 246kDa
- Gene ID
- 6712, 26915
- UniProt
- O15020
- Pathways
- Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
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