Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody

Catalog No. ABIN6938449

The Rabbit Monoclonal anti-Spectrin, Beta, Non-erythrocytic 2 antibody (Clone SPTBN2-2894R) (ABIN6938449) specifically detects Spectrin, Beta, Non-erythrocytic 2 in WB, IHC, FACS and ELISA. The antibody is reactive with Human samples.

Quick Overview for Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (ABIN6938449)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: SPTBN2-2894R

Product Details

Binding Specificity: AA 356-475

Purpose: Recombinant Spectrin beta III (SPTBN2) Antibody

Specificity: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), (ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Pancreas or Liver.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Alternative Name: SPTBN2

Background:

Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

HeLa cells. Pancreas or Liver.

Molecular Weight: 246kDa

Gene ID: 6712, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis