TCF4 antibody (AA 365-671)

Catalog No. ABIN6938516

This Mouse Monoclonal antibody specifically detects TCF4 in WB, ELISA, Coat and IHC (f). It exhibits reactivity toward Human.

Quick Overview for TCF4 antibody (AA 365-671) (ABIN6938516)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TCF4-1705

Product Details anti-TCF4 Antibody

Binding Specificity: AA 365-671

Specificity: Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells. Human bladder, colon or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Alternative Name: TCF4

Background: BHLHb19, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, PTHS, SEF2, SL3-3 enhancer factor 2, TCF4, Transcription factor 4,TCF4 (Transcription Factor 4)
Cellular localisation: Nucleus. Cytoplasm.

Molecular Weight: 60kDa

Gene ID: 6925, 605153

UniProt: P15884

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus