Transferrin Receptor antibody (AA 94-212)

Catalog No. ABIN6938531

This Mouse Monoclonal antibody specifically detects Transferrin Receptor in WB, ELISA, Coat and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Transferrin Receptor antibody (AA 94-212) (ABIN6938531)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TFRC-1817

Product Details anti-Transferrin Receptor Antibody

Binding Specificity: AA 94-212

Specificity: It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: TFRC

Background: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR,CD71 / Transferrin Receptor (TFRC) (Extracellular Domain)
Cellular localisation: Cell surface

Molecular Weight: 85-95kDa (monomer), 190kDa (dimer)

Gene ID: 7037, 529618

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis