TGFA antibody (C-Term)

Catalog No. ABIN6938541

The Mouse Monoclonal anti-TGFA antibody has been validated for IF, ELISA, IHC (f) and Coat. It is suitable to detect TGFA in samples from Rat.

Quick Overview for TGFA antibody (C-Term) (ABIN6938541)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: MF9

Product Details anti-TGFA Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Cross-Reactivity (Details): Human. Cow. Rat. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or Ramos cells. Testicular Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGFA

Background: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1,TGF-alpha (Transforming Growth Factor alpha)
Cellular localisation: Cytoplasmic and Extracellular (secreted)

Molecular Weight: ~6kDa

Gene ID: 7039, 170009

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway