TIMP2 antibody (N-Term)

Catalog No. ABIN6938576

This Mouse Monoclonal antibody specifically detects TIMP2 in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for TIMP2 antibody (N-Term) (ABIN6938576)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 3A4

Product Details anti-TIMP2 Antibody

Binding Specificity: AA 56-66, N-Term

Purpose: TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Antibody

Specificity: It recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Cross-Reactivity (Details): Human. Mouse. Rat. Guinea Pig. Rabbit. Cow. Zebrafish.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Background:

Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2

Colon, Endometrial, HeLa cells. Normal Placenta. Breast, Prostate or Ovarian Carcinoma.

Molecular Weight: 21kDa

Gene ID: 7077, 633514

UniProt: P16035

Pathways: cAMP Metabolic Process