ANO1 antibody (AA 904-986)

Catalog No. ABIN6938597

This Mouse Monoclonal antibody specifically detects ANO1 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for ANO1 antibody (AA 904-986) (ABIN6938597)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: DOG-1-1

Product Details anti-ANO1 Antibody

Binding Specificity: AA 904-986

Specificity: Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4 % vs. 94.7 %.

Cross-Reactivity (Details): Human. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide from human DOG-1 protein (aa 904-986), conjugated to a carrier protein. (

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: TMEM16A

Background: Anoctamin 1, Calcium Activated Chloride Channel, Discovered On Gastrointestinal Stromal Tumors Protein 1, TAOS2, ORAOV2, TMEM16A,DOG-1 / TMEM16A (Marker for Gastrointestinal Stromal Tumors)
Cellular localisation: Cell Surface and Cytoplasm

Molecular Weight: ~114kDa

Gene ID: 55107, 503074

UniProt: Q5XXA6