TYR antibody

Catalog No. ABIN6938695

The Mouse Monoclonal anti-TYR antibody (Clone T311) (ABIN6938695) specifically detects TYR in IHC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for TYR antibody (ABIN6938695)

Target: TYR (Tyrosinase (TYR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: T311

Product Details anti-TYR Antibody

Purpose: Tyrosinase (Melanoma Marker) Antibody

Specificity: Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant tyrosinase protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SK-MEL-13, SK-MEL-19, SK-MEL-30 or SK-MEL-37 cells. Human skin or melanoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: TYR

Background:

ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR

SK-MEL-13, SK-MEL-19, SK-MEL-30 or SK-MEL-37 cells. Human skin or melanoma.

Molecular Weight: 70-80kDa

Gene ID: 7299, 503555

UniProt: P14679