Uroplakin 1B antibody (AA 109-229)

Catalog No. ABIN6938733

The Mouse Monoclonal anti-Uroplakin 1B antibody has been validated for ELISA, Coat and IHC (f). It is suitable to detect Uroplakin 1B in samples from Human.

Quick Overview for Uroplakin 1B antibody (AA 109-229) (ABIN6938733)

Target: Uroplakin 1B (UPK1B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Uroplakin 1B antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: UPK1B-3102

Product Details anti-Uroplakin 1B Antibody

Binding Specificity: AA 109-229

Specificity: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.UPK1B is expressed by terminally differentiated urothelial cells.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Urinary Bladder.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Uroplakin 1B

Target: Uroplakin 1B (UPK1B)

Alternative Name: UPK1B

Background: Tetraspanin-20, Tspan-20, TSPAN20, UP1b, UPIb, UPK1B, Uroplakin-1b,Uroplakin 1B (Urothelial Differentiation Marker)
Cellular localisation: Cell Surface

Molecular Weight: 30kDa

Gene ID: 7348, 271580

UniProt: O75841