VEGFA antibody

Catalog No. ABIN6938743

This anti-VEGFA antibody is a Mouse Monoclonal antibody detecting VEGFA in IF and IHC (f). Suitable for Human.

Quick Overview for VEGFA antibody (ABIN6938743)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPM225

Product Details anti-VEGFA Antibody

Specificity: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Cross-Reactivity (Details): Human. Mouse. Rat. Rabbit. Dog.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human VEGF189 recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (4-8 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA

Background: MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA,VEGF (Vascular Endothelial Growth Factor)
Cellular localisation: Cytoplasmic, Cell Surface and Extracellular (Secreted)

Molecular Weight: 19-22kDa (reducing) and 38kDa-44kDa (non-reducing)

Gene ID: 7422, 73793

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling