Recombinant Villin 1 antibody (AA 179-311)

Catalog No. ABIN6938750

This Mouse Monoclonal antibody specifically detects Villin 1 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant Villin 1 antibody (AA 179-311) (ABIN6938750)

Target: Villin 1 (VIL1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RVIL1-1325

Product Details anti-Villin 1 Antibody

Binding Specificity: AA 179-311

Specificity: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa179-311) of human Villin protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: VIL1

Background: VIL1, Villin-1, Villin1,Villin (GI-Mucosal & Urogenital Brush Border Marker)
Cellular localisation: Cytoplasmic & Cell Surface

Molecular Weight: 93kDa

Gene ID: 7429, 654595

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization